Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BMI1

Cell type

Cell type Class
Neural
Cell type
Neural Stem Cells
MeSH Description
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Attributes by original data submitter

Sample

source_name
Neural stem cells
cell type
Hindbrain derived neural stem cells
genotype
H3.3K27
chip antibody
BMI1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone/protein-DNA complexes were isolated using antibodies. Libraries were constructed using the Rubicon Genomics DNA ThruPlex library preparation kit, catalog number R400427, in accordance with the manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
21359358
Reads aligned (%)
71.9
Duplicates removed (%)
22.7
Number of peaks
491 (qval < 1E-05)

hg19

Number of total reads
21359358
Reads aligned (%)
71.2
Duplicates removed (%)
23.0
Number of peaks
468 (qval < 1E-05)

Base call quality data from DBCLS SRA