Antigen

Antigen Class

TFs and others

Antigen

BMI1

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

source_name

Neural stem cells

cell type

Hindbrain derived neural stem cells

genotype

H3.3K27

chip antibody

BMI1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone/protein-DNA complexes were isolated using antibodies. Libraries were constructed using the Rubicon Genomics DNA ThruPlex library preparation kit, catalog number R400427, in accordance with the manufacturers instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

21359358

Reads aligned (%)

71.9

Duplicates removed (%)

22.7

Number of peaks

491 (qval < 1E-05)

hg19

Number of total reads

21359358

Reads aligned (%)

71.2

Duplicates removed (%)

23.0

Number of peaks

468 (qval < 1E-05)